B.S. (1978) Rice University
M.S. (1981) California Institute of Technology
Ph.D. (1984) California lnstitute of Technology
Systems biotechnology; metabolic engineering; biochemical engineering; Design and modeling of genetic and metabolic networks; Modeling, computer control and optimization of bioreactors.
Email: ksan@rice.edu
Phone: 713-348-5361
Office: E200K GRB Hall
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Ka-Yiu San
E. D. Butcher Professor in Bioengineering and Professor of Chemical Engineering
Our laboratory is mainly concerned with the development of new concepts and novel approaches of designing and manipulating metabolic routes using recombinant DNA technology to improve cellular activities and to achieve more advanced production systems. The main focus is on the development of a framework for the systematic analysis of the cellular responses in regulating its metabolic activities upon precise genetic perturbations. Understanding these responses is critical to design organisms and processes for efficient and reliable manufacturing of gene products from recombinant cells. - Metabolic Engineering - Cofactor Manipulations in Escherichia Coli
Most current metabolic engineering studies have focused on manipulations of enzyme levels and the effect of amplification, addition or deletion of a particular pathway. A newer focal area in our laboratory is the manipulation of cofactors, which play a major role in cell physiology and in the production of many important fermentation products. Additionally these cofactors are essential for a large class of enzymatic reactions. For example, the NADH/NAD+ pair serves as donor and/or acceptors of electrons in many biological redox reactions. The focus of our current research is to investigate the effect of different cofactor manipulations using cofactor-dependent products as model systems.
- Plant Metabolic Engineering
In a joint project with Dr. Shanks of Iowa State University and Dr. Gibson, our lab explores the area of metabolic engineering in plants. We are particularly interested in the metabolic engineering of the indole pathway and its subsequent effect on indole alkaloid biosynthesis in Catharanthus roseus hairy roots. C. roseus produces a wide variety of indole alkaloids including the anti-cancer drugs vincristine and vinblastine. Hairy roots have a number of advantages over cell cultures including increased genetic stability and substantially higher alkaloid content. Quantitative Systems Biotechnology: Recent rapid accumulation of enormous amount of genomic and pathway information provides a unique opportunity for the optimal design of genetic circuits and/or metabolic pathways to achieve a specific cellular behavior. Our laboratory is interested in the development of new analysis and design methodologies for the efficient utilization of these massive genomic and pathway knowledge.
- Quantitative Systems Biotechnology
Recent rapid accumulation of enormous amount of genomic and pathway information provides a unique opportunity for the optimal design of genetic circuits and/or metabolic pathways to achieve a specific cellular behavior. Our laboratory is interested in the development of new analysis and design methodologies for the efficient utilization of these massive genomic and pathway knowledge.
Selected Publications
Abstract
A. M. Sanchez, G.N. Bennett and K.-Y. San "Pathway Engineering of the Anaerobic Central Metabolic Pathway in Escherichia coli to Increase Succinate Yield and Productivity." Twenty First Conference on Biomedical Engineering Research in Houston (2004/02).
C. R. Dittrich, R.V Vadali, A. M. Sanchez, G.N. Bennett and K.-Y. San "Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB Pathways." Twenty First Conference on Biomedical Engineering Research in Houston (2004/02).
N.-R. Yun, K.-Y San, and George Bennett "Function of E. coli global redox sensor/ regulator, ArcAB, in anaerobic metabolic regulation and cellular responses." American Society for Microbiology (ASM) 104th General Meeting (2004/05).
Peercy, B., Shalel Levanon, S., Bennett, G.N., Cox, S.J., San, K.-Y. "Oxygen Sensing and Regulation of Cytochromes in E. coli." Gordon Research Conference on Theoretical Biology and Biomathematics (2004/06).
S.-. Hong, C. Peeble, J.V. Shanks, K.-Y. San, and S. I. Gibson "Expression of the Arabidopsis Feedback-insensitive Anthranilate Synthase Holoenzyme and Tryptophan Decarboxylase Genes in Catharanthus roseus Hairy Roots." ASPB's Annual Meeting (2004/07).
G.N. Bennett and K.-Y San "Cofactors in metabolic engineering of ester production." 228th ACS National Meeting (2004/08).
K.-Y. San, G.N. Bennett, S. Shalel Levanon, S. Cox, B. Peercy, J. V. Shanks, and R. Gonzalez "A genetic network driven metabolic flux analysis scheme." Metabolic Engineering Conference (2004/09).
H. Lin, A. Sanchez, G.N. Bennett and K.-Y. San "A metabolic engineering application: pathway design and engineering for enhancing succinate productivity in both aerobic and anaerobic fermentation processes." Metabolic Engineering Conference (2004/09).
J. V. Shanks, K.-Y San, S. I. Gibson, S.-B. Hong, E. Hughes, G. Sriram, C. Peebles, and G. Sander "An integrated metabolic engineering study of hairy roots for alkaloid production." Metabolic Engineering Conference (2004/09).
H. Lin, G.N. Bennett and K.-Y. San "Metabolic engineering of the central metabolic pathways of Escherichia coli: A systematic approach to study the metabolic response to multiple pathway manipulations for the production of fermentation products." Twenty First Conference on Biomedical Engineering Research in Houston (2004/02).
C. A. Peebles, E. Hughes, S.-B. Hong, S. Gibson, J. V. Shanks, and K.-Y. San "Metabolic engineering of indole alkaloid pathways In Catharanthus roseus hairy roots." Twenty First Conference on Biomedical Engineering Research in Houston (2004/02).
P.V. Vadlani, K.-Y. San, G.N. Bennett, Production of Natural Flavor Chemicals: Isoamyl Acetate Biosynthesis from Renewable Sugars Using Engineered Escherichia coli, Philadelphia, PA, November 12-16, 2008.
P.V. Vadlani, K.-Y. San, G.N. Bennett, Production of Platform Chemicals from Renewable Sugars: Low-Cost Succinic Acid Fermentation Using Engineered Escherichia coli, Philadelphia, PA, November 12-16, 2008.
14K.-Y. San, Manipulating and balancing reducing equivalents through metabolic engineering, Department of Chemical and Biomolecular Engineering, University of Maryland, October 14, 2008.
H. Lin, G.N. Bennett, and K.-Y. San "Characterization of a metabolically engineered Escherichia coli mutant designed for aerobic production of succinate: A systematic approach to study the metabolic responseto multiple pathway manipulations in the central metabolism." 227th ACS National Meeting (3/28-4/1).
K.-Y. San, S. Shalel Levanon, H. Lin, G. N.Bennett, S. Cox "Development of a metabolic network design and optimization framework incorporating implementation constraints." 227th ACS National Meeting (3/28-4/1).
S. Shalel Levanon, K.-Y. San, G. N. Bennett "Effect of oxygen on the E. coli global redox sensing/regulation networks and cellular responses." 227th ACS National Meeting (3/28-4/1).
A. M. Sanchez, I. Hussain, G. N. Bennett, K.-Y. San "Metabolic Engineering of NADPH availability on the productivity of poly(3-hydroxybutyrate) in Escherichia coli." 227th ACS National Meeting (3/28-4/1).
C. R. Dittrich, G.N. Bennett and K.-Y. San "Metabolic engineering of Escherichia coli to study the effects of ackA-pta and poxB deletions on the control of acetate production." 227th ACS National Meeting (3/28-4/1).
C. A. Peebles, E. Hughes, S.-B. Hong, S. Gibson, J. V. Shanks, and K.-Y. San "Metabolic engineering of the terpenoid indole alkaloid pathways in Catharanthus roseus hairy roots: The effects of terpenoid precursor feeding on transgenic ASab hairy roots." 227th ACS National Meeting (3/28-4/1).
K.-Y. San, G. N.Bennett, S. Shalel Levanon, S. Cox "Modeling of the oxygen Sensing/Regulatory Circuit in E. coli." 227th ACS National Meeting (3/28-4/1).
A. M. Sanchez, G.N. Bennett and K.-Y. San "Pathway engineering of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity." 227th ACS National Meeting (3/28-4/1).
N.-R. Yun, K.-Y. San, and George Bennett "Function of redox proteins, Ndh and Nuo, in anaerobic metabolic regulation and cellular responses in E. coli." ASM Conference on Integrating Metabolism and Genomics (4/30-5/3).
K.-Y. San, G.N. Bennett, R. Vadali, H. Lin and A. Sanchez "Co-factor engineering as a metabolic engineering tool to increase process productivity." Metabolic Engineering Conference.
H. Lin, G.N. Bennett, and K.-Y. San "Genetic reconstruction of the tricarboxylic acid cycle in Escherichia coli for the complete aerobic production of succinate through the glyoxylate bypass." 227th ACS National Meeting.
Article
Hong, S.-B., Peebles C.M., Shanks, J V., San, K.-Y., and Gibson, S. I. "Terpenoid indole alkaloid production by Catharanthus roseus hairy roots induced by Agrobacterium tumefaciens harboring rol ABC genes." Biotechnology and Bioengineering (2005).(In Press)
Peebles C.M., Hong, S.-B., Gibson, S. I. And Shanks, J V., San, K.-Y. "The transient effects of over-expressing anthranilate synthase alpha and beta subunits in Catharanthus roseus hairy root." Biotechnology Progress, 21 (2005): 1572-1576.
K.Y.San, B.E. Peercy, S.J. Cox, Shalel-Levanon, G.N. Bennett "A Kinetic Model of Oxygen's Regulation of Cytochrome Oxidase bo3 and Cytochrome Oxidase bd through the Sensor/Regulators FNR and Two Component ArcAB in Escherichia coli." Journal of Theoretical Biology, 242(3) (2006).
K.Y.San, H. Lin, N.M. Castro, G.N. Bennett "Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering." Applied Microbiology and Biotechnology, 71(6) (2006): 870-4.
K.Y.San, A.M. Sánchez, G.N. Bennett "Batch culture characterization and metabolic flux analysis of succinate producing Escherichia coli strains." Metabolic Engineering, 8 (2006): 209-226.
Dittrich, C. R., Vadali, R.V. Bennett, G. N., and San, K.-Y. "R Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate." Biotechnology Progress, 21 (2005): 627-631.
S¿nchez, A. M., Bennett, G. N., and San, K.-Y. "Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity." Metabolic Engineering, 7 (2005): 229-239.
Lin, H., Bennett, G. N., and San, K.-Y. "Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield." Metabolic Engineering, 7 (2005): 116-127.
Cox, S.J., Shalel Levanon, S., Bennett, G. N., and San, K.-Y. "Genetically constrained metabolic flux analysis." Metabolic Engineering (2005).(In Press)
Lin, H., Bennett, G. N., and San, K.-Y. "Genetic reconstruction of the aerobic central metabolism in Escherichia coli for the absolute aerobic production of succinate." Biotechnology and Bioengineering, 89 (2005): 148-156.
Lin, H., Bennett, G. N., and San, K.-Y. "Fed batch culture of a metabolically engineered Escherichia coli strain designed for high level succinate production and yield under aerobic conditions." Biotechnology and Bioengineering, 90 (2005): 775-9.
Vadali, R.V., Bennett, G. N., San, K. Y. "Enhanced lycopene productivity by manipulation of carbon flow to isopentenyl diphosphate in Escherichia coli." Biotechnology Progress, 21 (2005): 1558-1561.
Sanchez, A. M., Bennett, G. N., and San, K.-Y., "Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli." .(Submitted)
S¿nchez, A. M., Bennett, G. N., and San, K.-Y. "Effect of different levels of NADH availability on metabolic fluxes of Escherichia coli chemostat cultures in defined medium." Journal of Biotechnology, 117: 395-405.
Lin, H., Bennett, G. N., and San, K.-Y. "Effect of Sorghum vulgare phosphoenolypyruvate carboxylase and Lactococcus lactis pyruvate carboxylase coexpression on succinate production in mutant strains of Escherichia coli." Applied Microbiology and Biotechnology, 67: 515-23.
Dittrich, C. R., Vadali, R.V. Bennett, G. N., and San, K.-Y. "Characterization of the acetate-producing pathways in Escherichia coli." Biotechnology Progress, 21: 1062-7.
Peebles, C.A.M., Hughes, E., Shanks, J.V., and San, K.-Y., Transcriptional response of the terpenoid indole alkaloid pathway to the overexpression of ORCA3 along with jasmonic acid elicitation of Catharanthus roseus hairy roots over time, Metabolic Engineering, accepted (2008).
Peebles, C.A.M., Shanks, J.V., and San, K.-Y., The role of the octadecanoid pathway in the production of terpenoid indole alkaloids in Catharanthus roseus hairy roots under normal and UV-B stress conditions, submitted (2008)
Binder, B.Y.K., Peebles, C.A.M., Shanks, J.V., and San, K.-Y., The effects of UV-B stress on the production of terpenoid indole alkaloids in Catharanthus roseus hairy roots, Biotechnology Progress, accepted (2008).
Martinez, I., Zhu, J., Lin, H., Bennett, G.N., San, K.-Y., " Replacing Escherichia coli NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with a NADP-dependent enzyme from Clostridium acetobutylicum facilitates NADPH dependent pathways, Metabolic Engineering, accepted (2008).
Wong, M.S., Wu, S., Causey, T.B., Bennett, G.N., and San, K.-Y., ¿Reduction of acetate accumulation in Escherichia coli cultures for increased recombinant protein production¿, Metabolic Engineering, 10(2):97-108 (2008).
Chung, I. M., Ali, M., Chun, S. C., San, K.-Y., Peebles, C. A. M., and Ahmad, A., New catharanthusopimaranoside A And B from hairy root cultures of Catharanthus roseus, Chemistry of Natural Compounds, 44(4):458-462 (2008).
Dittrich, C.R., Bennett, G.N., San, K.-Y., Metabolic Engineering of the anaerobic central metabolic pathway in Escherichia coli for the simultaneous anaerobic production of isoamyl acetate and succinic acid, submitted (2008).
Chung, I.-M., Ali, M., Ahmad, A., Lee,O-K. Kim, J-H., Yoon, D-Y., Peebles, C.A.M., and San, K.-Y., Flavonoid Glucosides from the Hairy Roots of Catharanthus roseus, submitted (2008).
Peebles, C.A.M., Sander, G.W., Li, M., Shanks, J.V., and San, K.-Y., Five year maintenance of the inducible expression of anthranilate synthase in Catharanthus roseus hairy roots, Biotechnology and Bioengineering, accepted (2008).
Portle, S., Iadevaia1, S., San, K.-Y., Bennett, G.N., Mantzaris, N., Environmentally-modulated changes in fluorescence distribution in cells with oscillatory network dynamics, submitted (2008).
Wong, M.S., Causey, T.B., Bennett, G.N., and San, K.-Y., Engineering Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolymer Composition in E. coli, Biotechnology and Bioengineering, 99(4):919-28 (2008).
Singh, R., Vadlani, P.V., Harrison, M. L., Bennett, G.N., San, K.-Y., Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferase AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients Bioprocess and Biosystems Engineering, 31:299-306
K.Y.San, C.M. Peebles, S.B. Hong, S.I. Gibson, J.V. Shanks "The effect of terpenoid precursor feeding on Catharanthus roseus hairy root over-expressing the alpha or the alpha and beta subunits of anthranilate synthase." Biotechnology and Bioengineering, 93(3) (2006): 534-40.
K.Y.San, S.B. Hong, C.M. Peebles, J.V. Shanks, S.I., Gibson "Terpenoid Indole Alkaloid Production by Catharanthus roseus Hairy Roots induced by Agrobacterium tumefaciens harboring rol ABC genes." Biotechnology and Bioengineering, 93(2) (2006): 386-90.
K.-Y.. San, Y.C. Park, N.R., Yun, G.N.Bennett "Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene of Candida utilis ATCC 9950." J Ind Microbiol Biotechnol (2006).(In Press)
K.Y.San, G.N. Bennett, R. Singh, Y.T.Yang, Y.B. Liu "Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains." Biotechnology Progress, 22(3) (2006): 898-902.
K.Y.San, S.B. Hong, C.M. Peebles, J.V. Shanks, S.I. Gibson "Expression of the arabidopsis feedback-insensitive anthranilate synthase holoenzyme and tryptophan decarboxylase genes in Catharanthus roseus hairy roots." Journal of Biotechnology, 122 (2006): 28-38.
K.-Y.. San, J. Zhu, Shalel Levanson, G.N. Bennett "Effect of the global redox sensing/regulation networks on Escherichia coli and metabolic flux distribution based on C-13 labeling experiments." Metabolic Engineering, 8 (2006): 619-27.
K.Y.San, A.M. Sánchez, J. Andrew, I Hussein, G.N. Bennett "Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli”, Biotechnology Progress." Biotechnology Progress, 22(2) (2006): 420-5.
K.Y.San, H. Lin, S.J.Cox, Shalel Levanon, A. Sánchez, B. Peercy, G.N. Bennett "Development of a metabolic network design and optimization framework incorporating implementation constraints: a succinate production case study." Metabolic Engineering, 8 (2006): 46-57.
Berrios-Rivera, S. J., Sanchez, A. M., Bennet, G. N., San, K. Y. "Effect of different levels of nadh availability on metabolite distribution in Escherichia coli on minimal and complex media." (2003).(Submitted)
San, Ka-Yiu and Bennett, G.N., Meyers, R.A. (ed.) "Expression Systems for DNA Processes, in the Encyclopedia of Molecular Cell Biology And Molecular Medicine." VCH (2003).(In Press)
Hong, S.-B., Hughes, E.H., Shanks, J.V., San, K.-Y., and Gibson, S. I. "Role of the Non-Mevalonate Pathway in Indole Alkaloid Production by Catharanthus roseus Hairy Roots." Biotechnology Progress, 19 (2003): 1105 1108.
Berr¿os-Rivera, S. J., San, K.-Y., and Bennett, G. N. "The Effect of Carbon Sources and Lactate Dehydrogenase Deletion on 1,2-Propanediol Production in Escherichia coli." Journal of Industrial Microbiology and Biotechnology, 30 (2003): 34-40.
Lin, H., Bennett, G. N., and San, K.-Y. "Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: a study of the modified metabolic network based on metabolite profile, enzyme activity and gene expression profile." Metabolic Engineering (2005).(In Press)
Dittrich, C.R., San, K.-Y., and Bennett, G.N. "Cofactors in metabolic engineering of ester production, Proceedings of the American Chemical Society Symposium on Genetic Engineering in Flavor Chemistry: Applications and Potentials." ACS Press (2005).(In Press)
Yun, N.-R., San, K.-Y., and Bennett, G.N. "Effect of inactivation of adhE or pta-ackA in NADH dehydrogenase-deficient Escherichia coli on the pattern of anaerobic metabolic products." Journal of Applied Microbiology, 99 (2005): 1404¿1412.
Shalel Levanon, S., San, K.-Y., and Bennett, G. N. "Effect of oxygen on the E. coli ArcA and FNR regulation systems and metabolic responses." Biotechnology and Bioengineering, 89 (2005): 556-64.
Shalel Levanon, S., San, K.-Y., and Bennett, G. N. "Effect of oxygen, and ArcA and FNR regulators on the expression of genes related to the electron transfer chain and the TCA cycle in Escherichia coli." Metabolic Engineering (2005).(In Press)
S¿nchez, A. M., Bennett, G. N., and San, K.-Y. "Efficient succinate production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant." Biotechnology Progress, 21 (2005): 358-365.
Lin, H., Bennett, G. N., and San, K.-Y. "Effect of carbon sources differing in oxidation state and transport route on succinate production in metabolically engineered Escherichia coli." Journal of Industrial Microbiology & Biotechnology, 32 (2005): 87-93.
Shalel Levanon, S., San, K.-Y., and Bennett, G. N. "Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions." Biotechnology and Bioengineering (2005).(In Press)
Book
A Saterbak, L V McIntire, K San "Bioengineering Fundamentals." , ISBN-10: 0130938386 (January 2007).
Book chapter
San, K.-Y. and Bennett, G.N. "Expression Systems for DNA Processes." in the Encyclopedia of Molecular Cell Biology And Molecular Medicine, 4 (2004): 355-377.
Conference paper
R.V. Vadali, Y. Fu, G.N. Bennett and K.-Y. San "Effect of pathway manipulations on the production of lycopene In metabolically engineered Escherichia coli." 2003 Annual AIChE meeting (11.16-21.3).
A. M. Sanchez, R.V. Vadali, G.N. Bennett and K.-Y. San "Effects of NADH/NAD+ and NADPH/NADP+ cofactor pair manipulations in Escherichia coli." 2003 Annual AIChE meeting (11.16-21.3).
C. A. Peebles, E. Hughes, S.-B. Hong, S. Gibson, J. V. Shanks, and K.-Y. San "Metabolic engineering of indole alkaloid pathways In Catharanthus roseus hairy roots." 2003 Annual AIChE meeting (11.16-21.3).
A. M. Sanchez, G.N. Bennett and K.-Y. San "Cofactor and pathway engineering: A precise strategy to increase productivity of industrially useful compounds in Escherichia coli." 2003 Annual AIChE meeting (11.16-21.3).
S. Shalel Levanon, K.-Y. San, and G.N. Bennett "Analysis of E. coli global redox sensing/regulation networks and cellular responses." 2003 Annual AIChE meeting (11.16-21.3).
S. Shalel Levanon, K.-Y. San, G.N. Bennett and S. Cox "A data driven mathematical model of the O2 sensing/regulatory circuit in E. coli." 2003 Annual AIChE meeting (11.16-21.3).
G.N. Bennet and K.-Y. San "Regulating cofactors as a metabolic engineering strategy." Society for Industrial Microbiology (08.14.03).
S-B. Hong, E.H. Hughes, J.V. Shanks, K-Y. San, and S.I. Gibson "Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
H. Lin, G.N. Bennett, and K-Y. San "Production of succinate through metabolic engineering of Escherichia coli with coexpression of sorghum phosphoenolpyurvate carboxylase and lactococcus lactis pyruvate carboxylase." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
R.V. Vadali, E. Horton, K.-Y. San, F. B. Rudolph, and G.N. Bennett "Production of isoamyl acetate in genetic engineered Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
G. Bennett, S. Cox, S. Levanon, and K-Y. San "Metabolic flux analysis based on dynamic genomic information." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
E. Hughes, S-B. Hong, S. Gibson, J. Shanks, and K-Y. San "Metabolic engineering of indole alkaloid pathways in Catharanthus Roseus hairy roots." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
H. Lin, G.N. Bennett, and K-Y. San "Metabolic engineering of a succinate-producing mutant Escherichia coli strain to enhance succinate productivity." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
A. S¿nchez, K-Y. San, and G.N. Bennett "Manipulation of NADH availability as a metabolic engineering tool on cofactor dependent production systems." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
Y. Fu, R.V. Vadali, G.N. Bennett, and K-Y. San "Effect of pathway manipulations on the production of lycopene in metabolically engineered Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
A. S¿nchez, H. Lin, K.-Y. San, and G.N. Bennett "Effect of increased NADH regeneration on succinate production in Escherichia coli using NAD+-dependent formate dehydrogenase." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
R.V Vadali, E. Horton, K.-Y. San, F. B. Rudolph, and G.N. Bennett "Cofactor engineering of acetyl-CoA and its subsequent effect on metabolic fluxredistribution and the production of ester in Escherichia coli." Twentieth Conference on Biomedical Engineering Research in Houston (04.3-4.03).
E. Hughes, S.-B. Hong, S. Gibson, J. V. Shanks, and K.-Y. San "metabolic engineering of indole alkaloid pathways in catharanthus roseus hairy roots." 225th American Chemical Society National Meeting (03.23-7.03).
S.-B. Hong, E. H. Hughes, J. V. Shanks, K.-Y. San, and S. I. Gibson "Role of the non-mevalonate pathway in indole alkaloid production by Catharanthus roseus hairy roots." 225th American Chemical Society National Meeting (03.23-7.03).
R.V. Vadali, G.N. Bennett and K.-Y. San "Cofactor engineering of CoA/acetyl-CoA: A novel metabolic engineering technique to enhance process productivity in Escherichia coli." 2003 Annual AIChE meeting: 11.16-21.3.
K.-Y. San, S. Cox, G.N. Bennett "Metabolic flux analysis based on dynamic genomic information." (03.23-7.03).
H. Lin, G.N. Bennett and K.-Y. San "Increasing acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production in Escherichia coli." 225th American Chemical Society National Meeting (03.23-7.03).
R.V. Vadali, G.N. Bennett and K.-Y. San "Enhanced isoamyl acetate production in Escherichia coli upon manipulation of the acetyl-CoA node." 225th American Chemical Society National Meeting (03.23-7.03).
A. Sanchez, San, G.N. Bennett and K.-Y. San "Cofactor Metabolic Engineering: Comparative Study of the Effect of Increasing NADH Availability in E. coli Chemostat Cultures on Minimal and Complex Medium." 225th American Chemical Society National Meeting (03.23-7.03).
San, K.-Y. "Integration of undergraduate research in the BIOE curriculum at Rice University." (2005).
C. R. Dittrich, R.V Vadali, A. M. Sanchez, G.N. Bennett and K.-Y. San "Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB Pathways." 2003 Annual AIChE meeting (11.16-21.3).
H. Lin, G.N. Bennet and K.-Y. San "Metabolic engineering of the central metabolic pathways of Escherichia coli: A systematic approach to study the metabolic response to multiple pathway manipulations for the production of fermentation products." 2003 Annual AIChE Meeting (11.16-21.3).
S. Iadevaia, R.V Vadali, A. M. Sanchez, K.-Y. San and N. Mantzaris "Modeling and experimental studies for improved poly-3-hydroxybutyrate (PHB) production In E. coli." 2003 Annual AIChE meeting (11.16-21.3).
Refereed Article
Dittrich, C. R., Vadali, R.V. Bennett, G. N., and San, K.-Y. "Redistribution of Metabolic Fluxes in the Central Aerobic Metabolic Pathway of E. coli Mutant Strains with Deletion of the ackA-pta and poxB Pathways for the Synthesis of Isoamyl Acetate
." Biotechnology Progress (2004).(In Press)
Vadali, R.V., Horton, C.E., Rudolph, F.B., Bennett, G. N., San, K. Y. "Production of isoamyl acetate in ackA-pta and/or ldh mutants of Escherichia coli." Applied Microbiology and Biotechnology, 63 (2004): 698-704.
Hughes, E. H., Hong, S.-B., Gibson, S. I., Shanks, J. V., San, K.-Y. "Metabolic Engineering of the Indole Pathway in Catharanthus roseus Hairy Roots and Increased Accumulation of Tryptamine and Serpentine." Metabolic Engineering, 6 (2004): 268-276.
Lin, H., Vadali, R.V., Bennett, G. N., and San, K.-Y. "Increasing the acetyl-CoA pool in the presence of overexpressed phosphoenolpyruvate carboxylase or pyruvate carboxylase enhances succinate production in Escherichia coli." Biotechnology Progress, 20 (2004): 1599-1604.
Hughes, E. H., Hong, S.-B., Gibson, S. I., Shanks, J. V., San, K.-Y. "Expression of a Feedback-Resistant Anthranilate Synthase in Catharanthus roseus Hairy Roots Provides Evidence for Tight Regulation of Terpenoid Indole Alkaloid Levels." Biotechnology and Bioengineering, 86 (2004): 718-727.
Vadali, R.V., Bennett, G. N., San, K. Y "Enhanced Isoamyl acetate production upon manipulation of the acetyl-CoA node in Escherichia coli." Biotechnology Progress, 20(3) (2004): 692-7.
Berrios-Rivera, S. J., Sanchez, A. M., Bennett, G. N., San, K. Y. "Effect of different levels of NADH availability on metabolite distribution in Escherichia coli on minimal and complex media." Applied Microbiology and Biotechnology, 65(4) (2004): 426-432.
Lin, H., Bennett, G. N., and San, K.-Y. "Effect of Sorghum phosphoenolypyruvate carboxylase and Lactococcus lactis pyruvate carboxylase coexpression on metabolite production in Escherichia coli." Applied Microbiology and Biotechnology (2004).(In Press)
Shalel Levanon, S., San, K.-Y., and Bennett, G. N. "Effect of Oxygen on the E. coli ArcA and FNR Regulation Systems and Metabolic Responses." Biotechnology and Bioengineering (2004).(In Press)
Sanchez, A. M., Bennett, G. N., and San, K.-Y. "Effect of Different Levels of NADH Availability on Metabolic Fluxes of Escherichia coli Chemostat Cultures in Defined Medium." (2004).(Submitted)
Vadali, R.V., Bennett, G. N., San, K. Y. "Cofactor engineering of CoA/acetyl-CoA and its effect on metabolic flux redistribution in Escherichia coli." Metabolic Engineering, 6 (2004): 133-139.
Vadali, R.V., Bennett, G. N., San, K. Y. "Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli." Metabolic Engineering, 6 (2004): 294-299.
Chung, I.-M., Park, H.-Y., Ali, M., San, K.-Y., Peebles, C.A.M, Hong, S.-B., and Ahmad, A. "A new chemical constituent from the hairy root cultures of Catharanthus roseus." Bull. Korean Chem. Soc., 28(2) (2007): 229-234.
Peebles, C.A.M., Gibson, S.I., Shanks, J.V., and San, K.-Y "A study on the long-term maintenance of a transgenic Catharanthus roseus hairy root line." Biotech (2007).(In Press)
Portle, S., Causey, T. B., Wolf, K., Bennett, G. N., San, K.-Y., and Mantzaris, N. V. "Cell population heterogeneity in expression of a gene-switching network with fluorescent markers of different half-lives." J. Biotechnol, 128(2) (2007): 362-75.
Park, Y.C., Yun, N.R., San, K.-Y., and Bennett, G.N. "Characterization of alcohol dehydrogenase 1 and 3 from Neurospora crassa FGSC2489." Appl Microbiol Biotechnol, 76(2) (2007): 349-56.
Peebles, C.A.M., Gibson, S.I., Shanks, J.V., and San, K.-Y. "Characterization of an ethanol inducible promoter system in Catharanthus roseus hairy roots." Biotechnology Progress, 23 (2007): 1258-1260.
Chung, I.-M., Hong, S.-B., Peebles, C.A.M., and San, K.-Y. "Effect of the engineered indole pathway on accumulation of phenolic compounds in Catharanthus roseus hairy roots." Biotechnology Progress, 23 (2007): 327-32.
Chung, I.-M., Ali, M, Yang, Y.-M., Peebles, C.A.M, Chun, S.-C., Lee, S.-J., San, K. Y. and Ahmad, A "Identification of New Compounds from Catharanthus roseus Hairy Root Cultures." Bull. Korean Chem. Soc, 28 (2007): 1294-1298.
Chung, I.-M., Ali, M., Chun, S.-C., San, K.-Y., Peebles, C.A.M., and Ahmad, A. "New Sesquiterpene Glycosides from Cultures Hairy Roots of Catharanthus roseus." Chinese Journal of Chemistry, 25 (2007): 1695 -1699.
Zhu, J., Shalel Levanon, S., Bennett, G. N., and San, K.-Y "The YfiD protein contributes to the pyruvate formate-lyase flux in an E. coli arcA mutant strain." Biotechnology and Bioengineering, 97 (2007): 138-43.
Refereed Conference Paper
Cheryl R. Dittrich, Ka-Yiu San and George N. Bennett "Cofactors in metabolic engineering of ester production." .(Submitted)
Presentations
Invited Talk
"Efficient succinate production systems." Archer Daniels Midland Company (ADM), Decadal, Illinois. (January 19, 2006)
"Development of efficient succinate production systems." Ludwigshafen, Germany. (February 20, 2006) With G.N. Bennett.
"Metabolic engineering of Catharanthus roseus hairy roots." The Sixth Osong International Symposium, Cheong-ju, S. Korea. (November 1-2, 2007)
"Genetically constrained metabolic flux analysis." 106th ASM General Meeting, Orlando, FL. (May 21-25, 2006)
"Succinate and glyoxylate cycle." 2nd ASM Conference on Integrating Metabolism and Genomics (IMAGE2),, Montreal, Quebec, Canada. (April 30 - May 3, 2007)
"Highly efficient Escherichia coli systems for succinate production." 2006 World Congress on Industrial Biotechnology and Bioprocessing, Toronto, Canada. (July 11 - 14, 2006)
K.-Y. San, G.N. Bennett, Strategies to enhance recombinant protein production in Escherichia coli by reducing acetate accumulation, Society of Industrial Microbiology (SIM) Annual Meeting, San Diego, CA, August 10-14, 2008.
"Increasing intracellular reducing equivalents to enhance productivity in Escherichia coli." Third Conference on Renewable Resources and Biorefineries, Ghent, Belgium. (June 4-6, 2007) With G. N. Bennett.
"Pathway engineering for enhanced succinate production." 2006 Annual Meeting, Industrial Microbiology and Biotechnology Meeting, Baltimore, MD. (July 30-August 3, 2006)
"Design and analysis of highly efficient Escherichia coli succinate production systems." 62nd Southwest Regional Meeting of the ACS, Houston, TX. (October 19-22, 2006) With G.N. Bennett.
"Improvement of production of biochemicals through metabolic engineering." SIM Annual Meeting, Denver, CO. (July 29 - August 2, 2007) With G. N. Bennett.
"Pathway design and engineering to improve succinate productivity." The Second World Congress on Industry Biotechnology and Bioprocessing, Orlando, FL. (April 2005) With K.-Y. San, G. N. Bennett, A. M. Sanchez, and H. Lin.
"Design of biocatalyst for the synthesis of biorenewable-derived platform chemicals." NSF workshop - Design of Catalyst Systems for Biorenewables, Washington DC. (June 23-24, 2005) With K.-Y. San.
"Development of efficient Escherichia coli succinate production systems." Biochemical Engineering XIV, Harrison Hot Springs, BC, Canada. (July 10-14, 2005) With K.-Y. San, G. N. Bennett, A. M. Sanchez, and H. Lin.
"Development of efficient Escherichia coli succinate production strains." International Conference of Renewable Resources and Biorefineries, Ghent, Belgium. (September, 19-21, 2005) With K.-Y. San.
"Efficient succinate production systems." Cargill Inc.. (October 25, 2005.)
Other
M. Li, C.A.M. Peebles, J.V. Shanks, and K.-Y. San, Effects of Nitric Oxide on Growth and Terpenoid Indole Alkaloid Production In Catharanthus Roseus Hairy Root Culture, Philadelphia, PA, November 12-16, 2008.
"Mathematical Model of Oxygen Sensing in Escherichia coli." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With B. Peercy, S. S. Levanon, G.N. Bennett, S. Cox and K-Y. San.
M. Li, J. Zhu, R. Simonette, M.L. Harrison, S. Portle, G.N. Bennett, K.-Y. San, Construction and Analysis of Cell Population Heterogeneity of Negative Feedback Gene Auto-Regulatory Systems In Escherichia coli, Philadelphia, PA, November 12-16, 2008.
"Metabolic Network Engineering and Optimization of the Aerobic Central Metabolism of Escherichia coli for Achieving the Maximum Theoretical Yield of Succinate under Absolute Aerobic Conditions." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With H. Lin, G.N. Bennett and K-Y. San.
"Effect Of Oxygen on the Escherichia coli Global Redox Sensing/Regulation Networks and Cellular Responses." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With S.S. Levanon, K-Y. San and G.N. Bennett.
"Role of nuo Gene Cluster during Anaerobic Pyruvate Metabolism in Escherichia coli. Twenty Second Conference on Biomedical Engineering Research in Houston." Houston, Texas. (February10-11, 2005) With R. Singh, G.N. Bennett and K-Y. San.
"Characterization of a Gene-Switching Network." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With S. Portle, M. Harrison, K-Y.San, G.N. Bennett and N. Mantzaris.
"Development of a Genetic Network Driven Model for Sugar-Utilization Regulatory Systems." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With R. Peacock, J.V. Shanks, R. Gonzalez and K-Y. San.
"Bosynthesis of Ethyl Esters in Recombinant Escherichia coli Expressing Alcohol Acyltransferase from Strawberry and Thiolase from Clostridium acetobutylicum." 106th ASM General Meeting, Orlando, FL. (May 21-25, 2006) With Y-C. Park, L. Sullivan, G. N. Bennett.
"Transient Effect of Overexpressing As-alpha on Terpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With C. Peebles, S. Gibson, J.V. Shanks and K-Y. San.
""Characterization of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Production in Escherichia Coli.".". (Feb 8-9, 2007) With M. S. Wong, T. S. Causey, N. Mantzaris, G. N. Bennett.
"Batch Reactor Design and Optimization for Large-scale Succinate Production under Absolute Aerobic Conditions Using a Metabolically Engineered Aerobic Succinate Production System in Escherichia coli." Twenty Second Conference on Biomedical Engineering Research in Houston, Houston, Texas. (February10-11, 2005) With H. Lin, G.N. Bennett and K-Y. San.
"Construction of a Genetic Toggle Switch for Polyhydroxyalkanoate Production in Escherichia coli." 106th ASM General Meeting, Orlando, FL. (May 21-25, 2006) With T. B. Causey, S. Portal, N. Mantzaris, G. N. Bennett.
""Development of a Mechanistic Model for Sugar-Utilization Regulatory Systems.".". (February 8-9 2007) With R. Peacock, J. Zhu, J. V. Shanks, R. Gonzalez.
"Metabolic Flux Map of E. coli Ptsg Mutant and Wild Type Consuming Glucose/Xylose under Anaerobic Condition." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With M. Choudhary, Y. Dharmadi, R. Gonzalez, J.V. Shanks.
"Unique BIOE Undergraduate Courses at Rice University." Whitaker Foundation Biomedical Engineering Educational Summit Meeting, Leesburg, Virginia. (March, 2005) With K.-Y. San and A. Saterbak.
""The Transient Effect of Overexpressing ORCA3 Plus Jasmonic Acid Feeding in Catharanthus Roseus Hairy Roots.".". (Feb 8-9, 2007) With C. Peebles, S. I. Gibson, J. V. Shanks.
"Construction of a genetic toggle switch for the production of polyhydroxyalkanoates in Escherichia coli." ACS National Meeting, San Diego, CA. (March 2005) With T. B. Causey, S. Portle, K.-Y. San, and G. N. Bennett.
"User-Controlled Changes in Fluorescence Distribution in Cells with Oscillatory Genetic Network Dynamics." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With S. Portle, T. B. Causey, G. N. Bennett, N. V. Mantzaris.
""Manipulation of NADPH Availability in Escherichia coli by Genetic Modfication of the Glycolytic Pathway.".". (February 8-9, 2007) With I. Martinez, J. Zhu, G. N. Bennett.
"Increasing the in vivo Nadph Availability by Metabolic Engineering in Escherichia coli." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With I. Martinez, J. Zhu, H. Lin, G. N. Bennett.
"Role of nuo gene cluster during anaerobic pyruvate metabolism in Escherichia coli." ACS National Meeting, San Diego, CA. (March 2005) With R. Singh, G. N. Bennett, and K.-Y. San.
""User-Controlled Changes is Fluorescence Distribution Cells with Oscillatory Genetic Network Dynamics."." February 8-9, 2007.
"Metabolic Engineering of Artemisia Annua Hairy Roots." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With B.Y.K. Binder, C.A.M. Peebles, D.J. Morales,.
"Metabolic engineering of the terpenoid indole alkaloid pathways in Catharanthus roseus hairy roots: Overexpression of the terpenoid branch genes for geraniol 10-hydroxylase and 1-deoxy-D-xylulose 5-phosphate synthase." ACS National Meeting, San Diego, CA. (March 2005) With R..W. S. Peacock, C.A.M. Peebles, S. I. Gibson, J. V. Shanks, and K.-Y. San.
""Computational modeling and metabolic analysis of succinate production in engineered Escherichia Coli.".". (April 29-May 2 2007) With G. Bennett, A. Sanchez, H. Lin, S. Cox, Shalel Levanon, B. Peercy.
"Development of a Mechanistic Model for Sugar-Utilization Regulatory Systems." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With J. Zhu, R. Peacock, J.V. Shanks, R. Gonzalez.
"Metabolic engineering of the terpenoid indole alkaloid pathways in Catharanthus roseus hairy roots." ACS National Meeting, San Diego, CA. (March 2005) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks, K.-Y. San.
""Value-added Chemicals from Grain Sorghum: Isoamyl acetate and Succinic Acid Biosynthesis via Fermentation and Engineered Escherichia coli.".". (October 7-10, 2007) With P. V. Vadlani, G. N. Bennett.
"The Transient Effect of Jasmonic Acid Feeding along with Orca3 Overexpression in Catharanthus roseus Hairy Roots." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks,.
"The effect of overexpressing AS-alpha and DXS in Catharanthus roseus hairy roots on the terpenoid indole alkaloid pathway." ACS National Meeting, San Diego, CA. (March 2005) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks, K.-Y. San.
""Metabolic Engineering of the Terpenoid and Indole Pathways in Catharanthus Roseus Hairy Roots.".". (November 4-9, 2007) With Chrisite A.M. Peebles, Susan I. Gibson, Jacqueline V. Shanks.
"Chemostat culture characterization of metabolically engineered Escherichia coli mutant strains designed for optimal aerobic production of succinate: A study of the metabolic response to multiple pathway perturbations in aerobic central metabolism." ACS National Meeting, San Diego, CA. (March 2005) With H. Lin, G. N. Bennett, K.-Y. San.
"Metabolic Engineering of the Terpenoid and Indole Pathways in Catharanthus roseus Hairy Roots." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks.
""Complex Inducer-Controlled Behavior in Cell Populations with Oscillatory Genetic Network Dynamics.".". (November 4-9, 2007) With Stephanie Portle, George Bennett, Nikos V. Mantzaris.
"Effect of Operating Conditions on the Performance of Efficient Succinate-Producing Escherichia coli Strains." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With I. Martinez, G. N. Bennett.
"Effect of acetyl-CoA synthetase overexpression in Escherichia coli on metabolite production in the presence of glucose and acetate carbon sources." ACS National Meeting, San Diego, CA. (March 2005) With H. Lin, N. M. Castro, G. N. Bennett, K.-Y. San.
""Unraveling the Sugar Utilization Regulatory Systems in Escherichia coli.".". (November 4-9, 2007) With Y. Dharmadi, R. Gonzalez, J. V. Shanks.
"Effect of oxygen on the E. coli global redox sensing/regulation networks and cellular responses." ACS National Meeting, San Diego, CA. (March 2005) With S. Shalel Levanon, K.-Y. San, and G. N. Bennett.
"Manipulation of the Terpenoid Indole Alkaloid Pathway in Catharanthus roseus Hairy Roots." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks.
""The Effect of Uvb Light on Terpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots.".". (November 4-9, 2007) With B. Y. K. Binder, C. A. M. Peebles, J. V. Shanks.
"Utilization of a Genetic Toggle in the Control of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Composition in Recombinant Escherichia coli." 2006 Annual AIChE meeting, San Francisco, CA. (November 12-17, 2006) With M. Wong, T. B. Causey, N. V. Mantzaris, G. N. Bennett.
""Exploration of the Role of the Octadecanoid Pathway for Terpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots.".". (November 4-9, 2007) With C. A. M. Peebles, J. V. Shanks.
"Effect of gene inactivation of arcA and/or fnr in Escherichia coli on anaerobic metabolic pathway." 2005 International Meeting of the Microbiological Society of Korea, Whengsung, Kangwon-do. Korea. (May 12-13. 2005) With N.R. Yun, K-Y San, and G.N. Bennett.
"Effect of yfiD and poxB Gene Disruption on Microaerobic Pyruvate Catabolization and Metabolic Flux Distributions Based on C-13 Labeling Experiments." 2006 Annual AIChE meeting, San Francisco, CA,. (November 12-17, 2006) With J. Zhu, S. Shalel Levanon, G. N. Bennett.
""Development of a Mechanistic Model for Sugar-Utilization Regulatory Systems.".". (November 4-9, 2007) With J. Zhu, J. V. Shanks, R. Gonzalez.
"Integration of undergraduate research in the BIOE curriculum at Rice University." 2005 ASEE Annual Conference, Portland, OR. (June 12-16, 2005) With K.-Y. San.
""Increasing the In Vivo Availability of Reducing Equivalents by Metabolic Engineering in Escherichia coli.".". (November 4-9, 2007) With I. Martinez, J. Zhu, H. Lin, G. N. Bennett.
""Reduction of Acetate Production in Escherichia coli for Increased Recombinant Protein Production.".". (November 4-9, 2007) With M. S. Wong, S. Wu, T. B. Causey, G. N. Bennett.
"Effect of Gene Inactivation of adhE or pta-ackA in NADH Dehydrogenase-Deficient Escherichia coli on Anaerobic Metabolic Pathway." 2005 Annual Meeting & International Symposium of the Korean Society for Microbiology and Biotechnology (KMB), Seoul, Korea. (June 30, 2005 ¿ July 1, 2005) With N.R. Yun, K-Y San, and G.N. Bennett.
""Construction and Characterization of Negative Feedback Gene Regulation Systems in Escherichia coli.".". (November 4-9, 2007) With M. Li, J. Zhu, N. V. Mantzaris, G. N. Bennett.
"A Systems Biology Approach for Metabolic Engineering of E. coli Sugar-Utilization Regulatory Systems." Biochemical Engineering XIV, Harrison Hot Springs, BC, Canada. (July 10-14, 2005) With R. Gonzalez, Y. Dharmadi, J.V. Shanks, K.-Y. San.
""Metabolic Engineering of Pyruvate Metabolism by Combining Pathway Mutations and Manipulating Nadh Availability.".". (November 4-9, 2007) With J. Zhu, G. N. Bennett.
""Metabolic Engineering of Escherichia coli to Overproduce C4 Compounds.".". (November 4-9, 2007) With A. Lee, I. Martinez, G. N. Bennett.
"Metabolic Engineering of Two Pathways, the Indole and Terpenoid Pathways, in Catharanthus roseus hairy roots." Biochemical Engineering XIV, Harrison Hot Springs, BC, Canada. (July 10-14, 2005) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks, K.-Y. San.
"Effect of oxygen on the E. coli global redox sensing/regulation networks and metabolic flux distribution based on C-13 labeling experiments." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With J. Zhu, S. Shalel Levanon, G. N. Bennett2 and K.-Y. San.
"Characterization of the ecdysone agonist-inducible promoter and the ethanol inducible promoter in Catharanthus roseus hairy roots." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With C.A.M. Peebles, S. I. Gibson, J. V. Shanks, K.-Y. San.
"Metabolic Engineering of Artemisia annua hairy roots." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With C.A.M. Peebles, D. J. Morales, K.-Y. San.
"Overexpression of geraniol 10-hydroxylase and 1-deoxy-D-xylulose 5-phosphate synthase in Catharanthus roseus hairy roots." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With C.A.M. Peebles, R. Peacock, S. I. Gibson, J. V. Shanks, K.-Y. San.
"Dynamics of Cell Populations Carrying Gene-Switching Networks with Fluorescent Protein Markers of Different Half-Lives." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With S. Portle, T. B. Causey, K.-Y. San, G. N. Bennett and N. V. Mantzaris.
"Construction of a Genetic Toggle Switch for Polyhydroxyalkanoate Production in Escherichia coli." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With T. B. Causey, S. Portle, K.-Y. San, N. V. Mantzaris and G. N. Bennett.
"Population Distribution Patterns in Cells with Oscillatory Genetic Network Dynamics." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With S. Portle, M. L. Harrison, K.-Y. San, G. N. Bennett and N. V. Mantzaris.
"Development of a mechanistic model for sugar-utilization regulatory systems." Annual AIChE Meeting, Cincinnati, OH. (Oct 30-Nov 4, 2005) With R. Peacock, J. Zhu, J. V. Shanks, R. Gonzalez and K.-Y. San.
Seminar Speaker
"Metabolic and Cofactor Engineering." Tufts University, Medford, MA. (October 22, 2007)
K.-Y. San, Manipulating and balancing reducing equivalents through metabolic engineering, Department of Chemical and Biomolecular Engineering, University of Maryland, October 14, 2008.
"Pathway design and reconstruction for efficient succinate production in Escherichia coli." The University of California at Los Angeles, Los Angeles, CA. (April 6, 2007)
"Design and construction of microbial strains for efficient succinate production from renewable resources." Iowa State University, Ames, IO. (September 13, 2007)
"Metabolic pathway design and reconstruction for efficient succinic acid production." University of Massachusetts, . (November 17, 2005) With K.-Y. San.
Editorial Positions
Member of the Editorial Board, Metabolic Engineering, (2004).
Member of the Editorial Board, Journal of Electronic Biotechnology, (2004).
Member of the Editorial Board, Metabolic Engineering, (2003).
Member of the Editorial Board, Journal of Electronic Biotechnology, (2003).
Member of the Editorial Board, Metabolic Engineering, (2005).
Member of the Editorial Board, Journal of Electronic Biotechnology, (2005).
Member of the Editorial Board, Metabolic Engineering, (2007).
Member of the Editorial Board, Journal of Electronic Biotechnology, (2007).
Member of the Editorial Board, Metabolic Engineering, (2008).
Member of the Editorial Board, Electronic Journal of Biotechnology, (2008).
Member of the Editorial Board, The Open Systems Biology Journal, (2008).
Editor for Special Issue, Metabolic Engineering, (2008).
Member of the Editorial Board, Metabolic Engineering, (2006).
Member of the Editorial Board, Journal of Electronic Biotechnology, (2006).
Theses
Yea-Tyng Yang, PhD. "Metabolic Engineering and Flux Analysis of Escherichia coli." (1999).(Thesis or Dissertation Director)
Aasheesh Chhiber, Masters. "Application of Metabolic and Biochemical Engineering Techniques to Enhance Metabolic Flux Throughput and Improve Succinate Production in Escherichia coli." (2001).
Sharon Sauer, PhD. "Effect of Polar Functional Groups on the Phase Behavior of Amino Acids, Peptides and Polymers." (2001).(Co-Director)
, Ph.D. "Metabolic Engineering of Cofactors (NADH/NAD+) in Escherichia coli." (2002).(Thesis or Dissertation Director)
Eric Hughes, Ph.D. "Metabolic Engineering of Catharanthus Roseus Hairy Roots Using an Inducible Promoter System." (2003).(Thesis or Dissertation Director)
Eric Hughes, Ph.D. "Metabolic Engineering of Catharanthus Roseus Hairy Roots Using an Inducible Promoter System." (2003).(Thesis or Dissertation Director)
Ravishankar Vadali, Engineering. "1. CoA/acetyl-CoA: A novel strategy to enhance isoamyl acetate production in Escherichia coli." (2004).(Thesis or Dissertation Director)
Randeep M.S., "Director." (2005).(Committee Member)
Cheryl M.S., "Director." (2005).(Committee Member)
Ailen Ph.D., "Director." (2005).(Committee Member)
Henry Ph.D., "Director." (2005).(Committee Member)
Christie Peebles, PhD. "Metabolic Engineering of the Terpenoid Indole Alkaloid Pathway of Catharanthus roseus Hairy Roots." (2008).(Thesis or Dissertation Director)
Stephanie Portle, Ph.D. "Elucidating the Connection Between Cell Population Heterogeneity and Genetic Regulatory Architecture in Specific Artificial Networks." (2008).(Thesis or Dissertation Director)
Jin Yang, Ph.D. "Jin Yang." (Committee Member)
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